Effects of lentiviral delivery in different brain regions on transfection of rat dentate gyrus neurons
10.3760/cma.j.issn.1674-6554.2016.07.004
- VernacularTitle:不同脑区注射慢病毒对大鼠齿状回神经元的影响
- Author:
Lijuan ZHAO
;
Jiatian YU
;
Mengmeng SUN
;
Xincai MA
;
Deguang WANG
- Publication Type:Journal Article
- Keywords:
Lentivirus;
Dentate gyrus;
Hippocampus;
Medial entorhinal cortex
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2016;25(7):591-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of lentiviral delivery green fluorescent protein in different brain regions on the transfection of dentate gyrus neurons.Methods Lenti-pSyn-EGFP was stereotaxic injected into dentate gyrus (DG),Cornu Ammonis 1 (CAl) of hippocampus and medial entorhinal cortex (EC) of rats.All animals were perfused with 4% paraformaldehyde.Their brains were cut into 30 μm sections which were performed with Nissl fluorescent staining.Results The resuts showed that the GFP positive cells in DG area of DG,CA1,EC,DG-EC groups were (18.0± 1.0),(17.0±0.6),(17.3±0.6),(18.3±0.6) respectively,and there was no statistical difference among each group(P>0.05).Conclusion Lentivirus delivery in EC and CA1 both can transfect DG neurons.This is a useful method to transfect dentate gyrus neurons instead of injecting lentivirus into DG directly and avoid the lesion of DG.