Establishment of delay eyeblink conditioned reflex and the acquisition rule in cynomolgus monkey
10.3760/cma.j.issn.1674-6554.2016.03.002
- VernacularTitle:食蟹猴延迟性眨眼条件反射的建立及习得规律研究
- Author:
Rui LI
;
Fang YAN
;
Yingshan YANG
;
Chuanxu HONG
;
Guanren CHEN
;
Haiying WU
;
Zhaoyang TIAN
;
Jianfeng SUI
;
Bing WU
- Publication Type:Journal Article
- Keywords:
Delay eyeblink conditioning ( DEC);
Cynomolgus monkey;
Acquisition rate
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2016;25(3):198-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the method of establishing a model of delay eyeblink condi-tioning ( DEC) in no surgical cynomolgus monkey and analyze the related acquisition rule.Methods Using the special monkey chairs to fix 6 adult male cynomolgus monkeys,they were sitting on the chairs and keep-ing awake,and heads could rotate freely during the whole training session.In the experiment,the 6 monkeys were trained in DEC with a tone (500 ms,85 dB) as the conditioned stimulus (CS),paired with a corneal oxygen-puff (100 ms,5 psi) as the unconditioned stimulus (US).There were 120 trials per-session,and 2 sessions per-day.During the experiments,an infrared emitter/detector attached to spectacles for eyeblink re-cording,the data of conditioned response ( CR) and startle response ( SR) were analyzed offline.Result-s Among the 6 cynomolgus monkeys,4 of them completed all of the training process of DEC and 3 monkeys had successful acquisition of DEC.The average CR rate in the last training session reached ( 64.67 ± 2.00)%,(P<0.01);1 monkey only showed a high acquisition rate( 85.00%) at the first training session, and the low CR rate in the next training sessions with the average rate of 18.16%,suggesting that the DEC model was failed to established.The SR rates in 4 cynomolgus monkeys were low with average rate of 5.47%. Conclusion Although the DEC behavior training of the cynomolgus monkey is easy to be influenced by many inside or outside factors,DEC can be obtained under the condition of non surgery and proper braking.
