Heterodimerization of orexin 2 alpha and 2 beta receptor

VernacularTitle:食欲素受体2α与2β异源二聚体的研究
Author: Hai LU ; Yunlu JIANG ; Cuiqing ZHU ; Bo BAI
Publication Type:Journal Article
Keywords: Orexin receptor; Fluorescence resonance energy transfer; Bioluminescence resonance energy transfer; Heterodimerization
From: Chinese Journal of Behavioral Medicine and Brain Science 2016;25(4):310-314
CountryChina
Language:Chinese
Abstract: Objective To explore the possibility of heterodimerization between orexin type 2α receptor (OX2αR) and orexin type 2β receptor (OX2βR).Methods Using confocal laser scanning microscope,enzyme linked immunosorbent assay (ELISA),fluorescence resonance energy transfer (FRET) and Bioluminescence resonance energy transfer (BRET) to study the interaction between OX2αR and OX2βR.Result Confocal laser scanning microscope and ELISA showed that OX2αR and OX2βR were both expressed in the cytoplasm.The FRET demonstrated that the signal of the experimental group (OX2αR-YFP+ OX2βR-CFP) was significantly stronger than that of control group (YFP+OX2βR-CFP).The BRET value of the experimental group (OX2αR-YFP+OX2βR-Rluc,mBRET ratio was 65± 15) was higher than that of control group (YFP+ OX2βR-Rluc/OX2αR-YFP+Rluc,mBRET ratio was 10±5) (P＜0.05).Conclusion There are heterodimerization between mOX2αR and mOX2βR.