The establisment of human craniopharyngioma xenografts in chick chorioallantoic membrane
10.3969/j.issn.1002-0152.2015.11.003
- VernacularTitle:颅咽管瘤鸡胚绒毛尿囊膜移植瘤模型的建立
- Author:
Xiaorong YAN
;
Dezhi KANG
;
Yuanxiang LIN
;
Jun PAN
;
Xiyue WU
;
Jie ZHOU
;
Changzhen JIANG
;
Songtao QI
- Publication Type:Journal Article
- Keywords:
Craniopharyngioma;
Chick chorioallantoic membrane;
Angiogenesis;
Proliferation
- From:
Chinese Journal of Nervous and Mental Diseases
2015;(11):651-655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the xenotransplanted tumor model of Craniopharyngioma in chick chorioallan?toic membrane (CAM) and detect the angiogenesis ability, microvessel density (MVD) and cell proliferation of the xeno?graft. Method Craniopharyngioma tissues from surgical craniopharyngioma patients were transplanted on the CAM. An?giogenesis assay was performed and the MVD and PCNA were evaluated using immunohistochemistry following the trans?plantation. Results The tumor formation rate of adamantinomatous craniopharyngioma (ACP) and squamous papillary cra?niopharyngioma (SPCP) was 47.14% and 43.33%, respectively. There was no significant difference in tumor formation rate between ACP and SPCP(χ2=0.123,P=0.726). The CAM angiogenesis, MVD and expression of PCNA were higher in ACP than in SPCP. The expression of PCNA was positively correlated with MVD (Pearson r=0.639,P<0.001) and CAM assay score (Spearman r=0.490,P=0.001 ) in CP. Conclusion The model of human craniopharyngioma can be es?tablished in the CAM. The angiogenesis of the xenograft in the CAM can be evaluated and the craniopharyngioma xeno?graft of CAM possesses a new blood circulation and cell proliferation ability.
