Investigation of Growth-Promoting Properties of the Culture Media from Different Sources for Drug Myco-plasma Test
- VernacularTitle:不同来源药品支原体检查用培养基的促生长能力研究
- Author:
Hongda ZHAO
;
Wen XIE
;
Wenping FAN
;
Xinyue XIAO
;
Shufang MENG
- Publication Type:Journal Article
- Keywords:
Mycoplasma test;
Media;
Chinese pharmacopoeia;
Growth-promoting properties
- From:
China Pharmacist
2015;18(12):2062-2066
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the growth-promoting properties and applicability of the mycoplasma test culture media pre-scribed in Chinese pharmacopoeia, and provide reference for the standardization of the drug mycoplasma test method. Methods: Re-spectively using four quantitative detection methods including sensitivity, degree of color change, colony counts and colony diameter, and mycoplasma media widely used in the world as the reference media, the growth-promoting properties of 4 batches of mycoplasma broth and 4 batches of arginine mycoplasma broth from four domestic manufacturers were investigated. Results:The results of sensitivity assay and absorbance detection showed that all the media inoculated with below 100 CFU test microorganisms exhibited visible color change. Furthermore, the results of color change degree and colony diameter showed that there were significant differences among the media products from different manufacturers(P<0. 01). Conclusion:Mycoplasma broth and arginine mycoplasma broth both can sup-port the growth of below 100 CFU test microorganisms. Due to the difference in the growth-promoting properties among the media prod-ucts from different manufacturers, the drug mycoplasma test workers should use more sensitive methods to examine the applicability of the media.
