Determination of Related Substances in Erythromycin Ophthalmic Ointment
- VernacularTitle:红霉素眼膏有关物质的测定
- Author:
Xiaodong ZHAO
;
Rong FU
;
Yu ZOU
- Publication Type:Journal Article
- Keywords:
Erythromycin ophthalmic ointment;
Related substance;
Extraction method;
HPLC with gradient elution
- From:
China Pharmacist
2015;18(12):2168-2170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of related substances in erythromycin ophthalmic ointment. Methods:The extraction method was established and optimized. An HPLC gradient elution method was used for the determination of related substances in erythromycin ophthalmic ointment. Results: One step extraction with PBS (pH 7.0) - methanol(1: 1) had promising effect. After the method validation, it was proved that the method could be used to determine the related substances in eryth-romycin ophthalmic ointment. Conclusion:The method established in the paper provides a better analytical extraction and determina-tion method for the quality control of erythromycin ophthalmic ointment.
