Preliminary Study on Extraction and Separation of Anti-tumor Ingredients from Fresh Gekko Swinhonis
- VernacularTitle:鲜无蹼壁虎抗肿瘤活性成分提取分离的初步研究
- Author:
Hongxia LI
;
Jiangong KANG
;
Shizhuang ZHANG
- Publication Type:Journal Article
- Keywords:
Fresh Gekko swinhonis;
Anti-tumor active ingredient;
Extraction and separation
- From:
China Pharmacist
2015;(11):1841-1843
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the extraction and separation of anti-tumor active ingredients from fresh Gekko swinhonis. Meth-ods:The extraction was carried out with ethanol at different concentrations by various methods. The extract was purified by HPLC after deproteinization and degreasing using a repeated freezing and thawing method. MTT was used to detect the antitumor activity of the ex-tract. Results:The A value of extraction process groupⅢwith 85% ethanol ultrasonic extraction was (0. 548 ± 0. 045) with the high-est tumor inhibition rate, and compared with the control group, the difference was significant (P<0. 05). Conclusion:The results of pharmacodynamics show that the optimum extraction technology is ultrasonic extraction with 85% ethanol.
