Effects of MicroRNA-154 on Apoptosis of Hepatic Stellate Cells
- VernacularTitle:MicroRNA-154对大鼠肝星状细胞HSC-T6凋亡的影响
- Author:
Ying ZHANG
;
Hong ZHANG
;
Rui PENG
;
Danyun WEI
- Publication Type:Journal Article
- Keywords:
MicroRNA-154;
Hepatic stellate cells;
Transfection;
Apoptosis
- From:
China Pharmacist
2015;(11):1859-1863
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-154 on the apoptosis of hepatic stellate cells (HSC-T6). Methods:Hepat-ic stellate cells (HSC-T6) were transfected with miR-154 mimics and miR-154 inhibitor, and the cells were trandfected with mimics NC and inhibitor NC as the negative control. The effects of miR-154 on the proliferation of HSC-T6 were detected at different time points by CCK-8. A flow cytometry with double staining of Annexin and PI was used to detect the cell cycle and apoptosis rate of HSC-T6. Results:The proliferation ability of the cells was increased,the apoptosis rate was decreased significantly, and the proportion of cells in G0/G1 phase were decreased, and those in G2/M phase were increased significantly after transfected with miR-154 mimics. The proliferation ability of the cells was decreased,the apoptosis rate was increased significantly, and the proportion of cells in G0/G1 phase were increased, and those in G2/M phase were decreased significantly after transfected with miR-154 inhibitor. Conclusion:MiR-154 can promote the proliferation of HSC-T6 and inhibit the apoptosis of HSC-T6.
