Determination of Content and Entrapment Efficiency of VEC-5 Liposomes by HPLC
- VernacularTitle:HPLC法测定VEC-5脂质体药物含量及包封率
- Author:
Bingxue SUN
;
Guoqing CHEN
;
Rui GENG
;
Haifeng LUO
;
Xinghong ZHAO
;
Yan CHEN
- Publication Type:Journal Article
- Keywords:
VEC-5;
Liposomes;
HPLC;
Content;
Entrapment efficiency
- From:
China Pharmacist
2015;18(10):1642-1645
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of content and entrapment efficiency of HIV-1 virus in-fection factor Vif inhibitor VEC-5 liposomes. Methods:VEC-5 liposomes were prepared by a method of freeze-drying and reconstruc-tion. The separation of free drug from the liposomes was achieved by ultracentrifugation, and an HPLC method was used to determine the content and entrapment efficiency of VEC-5 liposomes. Results:The linear range of VEC-5 was 20-100 μg·ml-1(r=0. 999 0). The average recovery was 100. 25% and RSD was 0. 93%(n=9). The content of three batches of VEC-5 liposomes was 98. 63%, 100. 43% and 102. 65%, respectively within the range of 90%-110%, and the entrapment efficiency was 94. 89%, 93. 68% and 94. 56%, respectively, which was above 90%. Conclusion:The method is accurate and reliable, which can be used to determine the content and entrapment efficiency of VEC-5 liposomes.
