Establishment of an HPLC-MS/MS Method for the Determination of Lamotrigine in Human Plasma and its Clinical Application
- VernacularTitle:人血浆中拉莫三嗪浓度测定的 HPLC-MS/MS 方法建立及临床应用
- Author:
Leting ZHU
;
Runxin CHEN
;
Li YANG
;
Zhigang ZHAO
;
Ming ZHAO
;
Shenghui MEI
- Publication Type:Journal Article
- Keywords:
HPLC-MS/MS;
Lamotrigine;
Therapeutic drug monitoring
- From:
China Pharmacist
2015;18(10):1701-1705
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC-MS/MS method for the determination of lamotrigine ( LTG) in human plasma to be applied in the clinical therapeutic drug monitoring. Methods:LTG was analyzed on a Kromasil C8 (50 mm × 2. 1 mm,5μm) column. Methanol and water (both containing 0. 1% formic acid) was used as the mobile phase with gradient elution. The flow rate was 0. 6 ml ·min-1 at the column temperature of 40℃. The ion transitions under an ESI positive model were performed at m/z 256. 0>211. 0 and m/z 264. 1>154. 0 for LTG and ticlopidine (internal standard, IS), respectively. Results: The calibration curve of LTG was linear within the range of 0. 02-2 μg · ml-1 . The recoveries of LTG at three quality control levels were within the range of 91. 94%-100. 28%. LTG was stable under all tested conditions and the dilution (the dilution factor was 10) had no influence on the accuracy and precision of LTG determination. Conclusion:The HPLC-MS/MS method for the determination of LTG developed in the study is accuracy, stable and convenient, and is applicable in the clinical therapeutic drug monitoring of LTG.
