Determination of Feruloyltyramine in Pothi Chinensis Herba by HPLC
- VernacularTitle:HPLC法测定石柑子中阿魏酰酪胺的含量
- Author:
Wenhui LUO
;
Buming LIU
;
Xiaoli BI
;
Lishi PENG
;
Hongcong QIU
- Publication Type:Journal Article
- Keywords:
Pothi chinensis herba;
Feruloyltyramine;
HPLC
- From:
China Pharmacist
2015;18(10):1790-1792
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC method for determining feruloyltyramine in Pothi chinensis herba. Methods: The HPLC determination was performed on a Thermo ODS-2 Hypersil column(250 mm × 4. 6 mm, 5 μm) with isocratic elution of metha-nol-0. 4% phosphoric (35:65) as the mobile phase. The flow rate was 1. 0 ml·min-1 . The column temperature was at 25℃, and the detection wavelength was set at 318 nm. Results: Feruloyltyramine showed good linearity,and the recovery was 99. 40% with RSD of 1. 44% (n =9). Conclusion: The method is quick, simple and reproducible, which can be used to control the quality of Pothi chinensis herba.
