Effect of Traditional Chinese Medicine Practolol Oral Liquid on Water Load Rat Model
- VernacularTitle:中药心得宁口服液对水负荷大鼠模型的影响
- Author:
Jingsheng ZHAO
;
Jun YANG
;
Weiguo YI
;
Hong LIU
;
Zhengchen ZHANG
- Publication Type:Journal Article
- Keywords:
Practolol oral liquid;
Rat;
Water load model;
Furosemide tablets;
Qiliqiangxin capsules
- From:
China Pharmacist
2015;18(10):1810-1813
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of practolol oral liquid on the rat model of water load. Methods: Totally 70 qualified rats were selected and randomly divided into seven groups with 10 ones in each, and the rats were with intragastric administra-tion respectively by practolol oral liquid (20,10 and 5 ml·kg-1 , respectively diluted 20 ml, 10 ml and 5 ml to 20 ml liquid with dis-tilled water), furosemide tablets suspension (0. 033 5 mg·ml-1), Qiliqiangxin capsule suspension(0. 03 g·ml-1) and distilled wa-ter with the same voulme( model) . The rats were placed in metabolism cages. After the administration, the urine volume was recorded every 1 h for 5 hours, the total volume of urine was collected during the 5 h, and the concentration of Na+, K+ and Cl- in the urine was measured. Results:Compared with that in the blank group, the urine volume in 2 h, 3 h and 5 h after the administration in the model group was significantly increased(P<0. 01), the total urine volume was also increased significantly(P<0. 01), the K+ con-centration was significantly reduced in the urine(P<0. 05), and there was an increasing trend of Na+ and Cl- concentration in the u-rine, which indicated that the water load model was made successfully. Compared with the model group, furosemide could significantly increase the urine volume in 1 h, 2 h, 3 h, 4 h and 5 h after the administration, and the total urine volume was increased as well ( P<0. 01). Qiliqiangxin capsule group could significantly increase the K+ concentration in the urine(P<0. 05), and furosemide could significantly reduce the K+ concentration in the urine (P<0. 05). High-dose practolol oral liquid could significantly increase the urine volume in 5 h after the administration and the Na+ and Cl- concentration(P<0. 01), mid-dose practolol oral liquid could significantly increase the urine volume in 3 h and 5 h after the administration and the total urine volume (P<0. 05), and improve the Cl- concen-tration in the urine (P<0. 05), and the Na+ and Cl- concentration in low-dose practolol oral liquid group was decreased significantly (P<0. 01). Compared with Qiliqiangxin capsule group, the high, medium and low-dose practolol oral liquid showed no significant in-fluence on the urine volume(P>0. 05), high-dose practolol oral liquid could significantly increase the Na+ concentration in the urine (P<0. 01), medium and low-dose practolol oral liquid could significantly increase the Na+ concentration in the urine(P<0. 05), low-dose practolol oral liquid could significantly reduce the K+ concentration(P<0. 01), high and medium-dose practolol oral liquid could significantly increase the Cl- concentration in the urine (P<0. 01), and low-dose practolol oral liquid could significantly reduce the Cl- concentration in the urine (P<0. 01) . Compared with the high-dose practolol oral liquid group, the influences of medium and low-dose practolol oral liquid showed no significant difference in the urine volumes(P>0. 05), however, the total urine volume could significantly increase(P<0. 01), and the medium and low-dose practolol oral liquid could significantly reduce the Na+ and Cl- con-centration in the urine(P<0. 01). Conclusion:Practolol oral liquid has significant diuretic effect in a dose-dependent manner.
