Simultaneous Determination of Six Flavonoid Ingredients in Shuxuening Injection by Quantitative Analysis of Multi-components by Single-marker
- VernacularTitle:一测多评法同时测定舒血宁注射液中6种黄酮类成分
- Author:
Fang ZHANG
;
Hongli GAO
- Publication Type:Journal Article
- Keywords:
QAMS;
Relative correction factor;
HPLC;
Shuxuening injection;
Rutin;
Narcissus glycosides
- From:
China Pharmacist
2015;18(10):1652-1656
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To simultaneous determine six flavonoids in Shuxuening injection by quantitative analysis of multi-compo-nents by single marker ( QAMS) and examine the durability and system suitability of the analysis. Methods:Shuxuening injection was the study object and rutin was used as the internal material, the relative correction factor ( f) between rutin and narcissus glycosides, quercetin-3-O-β-D-glucosyl (1-2)-α-L- rhamnoside, kaempferol-3-O-β-D-glucose group (1-2)-α-L-rhamnopyranoside, quercetin-3-O-[2-O-(1-O)-p-coumaric acid-β-D-glucose-yl]-α-L-rhamnoside, kaempferol-3-O-[2-O-(6-O)-on coumaroyl-β-D-glucose-based]-α-L-rhamnosides was respectively established. The result differences between the internal standard method and external standard method were compared, and the internal standard method was re-validated. Results: The contents of the six flavonoids in Shuxuening injection were calculated by the correction factors. Compared with those of the external standard method, the differences in the contents of the method were small( RSD<5%) . Conclusion:QAMS can be used as a simple and accurate quantitative quality evaluation meth-od for the determination of a variety of ingredients in Shuxuening injection, which provides reference for the subsequent study on quality standard.
