Improvement of Assay Method for Mori Folium in Chinese Pharmacopoeia
- VernacularTitle:《中国药典》中桑叶含量测定项下方法的改进
- Author:
Juhua MAO
;
Weiying WANG
;
Zhangjin CHEN
;
Wei XUE
- Publication Type:Journal Article
- Keywords:
Mori Folium;
Rutin;
Isoquercitrin;
Assay
- From:
China Pharmacist
2015;(8):1397-1399
- CountryChina
- Language:Chinese
-
Abstract:
To optimize the extract method and chromatographic conditions for the determination of rutin in Mori Folium in Chinese pharmacopoeia. Methods:The HPLC analysis was performed on a ZORBAX SB-C18 (250 mm × 4. 6 mm, 5 μm) column. The mobile phase was acetonitrile-0. 2% phosphorice acid solution with gradient elution and the flow rate was 1. 0 ml·min-1 . The de-tection wavelength was 354 nm, and the column temperature was 30 ℃. Results: The content of rutin determined by the method in Chinese pharmacopoeia was actually the total contents of rutin and isoquercitrin, while the optimized method could separate the two components effectively, and the good linearity of rutin and isoquercitrin was within the range of 2.76-27.60 μg·ml-1(r=0.999 9) and 4. 74-47. 39 μg·ml-1(r=0. 999 8), respectively, and the average recovery was 100. 31% (RSD=0. 83%) and 100. 32%, re-spectively (RSD=1. 04%, n=6). Conclusion:The optimized method is simple, stable and reproducible,which can be used in the quality control of Mori Folium.
