Improvement of Quality Standard for Pingyou Granules
- VernacularTitle:平疣颗粒的质量标准提升研究
- Author:
Hua SU
;
Xin LIAO
;
Liye QIAO
;
Yin LU
;
Haixiang REN
- Publication Type:Journal Article
- Keywords:
Pingyou granules;
Quality standard;
TLC;
Paeoniae radix;
Carthamus tinctorius;
HPLC;
Peoniflorin
- From:
China Pharmacist
2015;(8):1305-1308
- CountryChina
- Language:Chinese
-
Abstract:
To establish the quality standard for Pingyou granules. Methods: Paeoniae radix, Carthamus tinctorius, Coicis semen and Nutgrass galingale rhizome were qualitatively identified by TLC. The content of peoniflorin was determined by HPLC. The separation was performed on a Lichrospher-C18 (250 mm × 4. 6 mm, 5 μm) column with the mobile phase of methanol-acetic acid (24∶76). The detection wavelength was 232nm. Results:Paeoniae radix, Carthamus tinctorius, Coicis semen and Nutgrass galingale rhizome could be identified by TLC without any interference from the negative control. The linear range of peoniflorin was 6. 560-104. 920 μg·ml-1(r=0. 999 9) with the average recovery of 98. 77%(RSD=2. 73%,n=9). Conclusion:The qualitative identifi-cation is specific and reproducible, and the quantitative method is simple, accurate and reliable, which can be used in the quality con-trol of Pingyou granules.
