Study on HPLC Fingerprint of Polygonum Cuspidatum Dispensing Granules
- VernacularTitle:虎杖配方颗粒的HPLC指纹图谱研究
- Author:
Jun LIANG
;
Jiangping ZHENG
;
Liangyong HUANG
- Publication Type:Journal Article
- Keywords:
Polygonum cuspidatum;
Dispensing granules;
HPLC;
Fingerprint;
Polydatin;
Emodin
- From:
China Pharmacist
2015;(4):578-582
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC fingerprint for Polygonum cuspidatum dispensing granules. Methods:The HPLC fin-gerprint of 12 batches of Polygonum cuspidatum from different manufacturers were determined. The analysis was performed on a Waters SunFire C18 column(250 mm × 4. 6 mm,5μm)with acetonitrile as the mobile phase A and 0. 05% phosphoric acid solution as the mo-bile phase B with gradient elution. The flow rate was 1. 0 ml·min-1 ,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10μl. Results:The results were calculated according to“similarity evaluation system for tradi-tional Chinese medicine chromatographic fingerprint”nominated by CFDA combined with the analysis of the HPLC fingerprints. Totally 14 common peaks with similarity above 0. 98 were found in the HPLC fingerprint of Polygonum cuspidatum,including the peak respec-tively for polydatin and emodin. Conclusion:The method can provide more information for the quality control of Polygonum cuspidatum dispensing granules.
