Simultaneous Determination of Two Components in Chlorphenamine Maleate and Ephedrine Nasal Drops by HPLC
- VernacularTitle:HPLC法同时测定扑麻滴鼻液中2成分的含量
- Author:
Li JIN
;
Jianli ZHOU
;
Xiaodan ZHANG
;
Xiaoyong WU
;
Ying BU
;
Zhuoliang GU
- Publication Type:Journal Article
- Keywords:
Chlorphenamine maleate and ephedrine nasal drops;
HPLC;
Ephedrine hydrochloride;
Chlorphenamine maleate
- From:
China Pharmacist
2015;(4):687-689
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the simultaneous determination of chlorphenamine maleate and ephedrine hydro-chloride in chlorphenamine maleate and ephedrine nasal drops by HPLC. Methods:An HPLC method was performed on a column of Purospher STAR RP18(150 mm × 4. 6 mm,5 μm)with the mobile phase of acetonitrile -potassium dihydrogen phosphate buffer(pH 3. 0 ± 0. 1)(18:82 v/v)at the detection wavelength of 210 nm(adjusting the flow rate and column temperature to 1. 0 ml·min-1 and 35℃,respectively). The injection volume was 20 μl. Results:A good linear relationship was established between the peak response and the concentration of ephedrine hydrochloride and chlorphenamine maleate over the range of 19. 81-118. 85μg·ml-1(r=0. 999 6) and 6. 21-37. 25 μg·ml-1(r=0. 999 8),respectively. The mean recovery of ephedrine hydrochloride and chlorphenamine maleate was 100. 39%(RSD=0. 69%,n=9)and 100. 11%(RSD=0. 60%,n=9),respectively. Conclusion:The proposed method shows high repeatability,good durability and promising accuracy. It can be employed for the determination of two components in chlorphena-mine maleate and ephedrine nasal drops.
