A study of in vivo two-photon imaging of cerebravascular CO2 reactivity in mice
10.3936/j.issn.1002-0152.2015.05.001
- VernacularTitle:活体双光子成像观察小鼠脑血管CO2反应性及其机制
- Author:
Mingyue LI
;
Tengteng WU
;
Sai ZHANG
;
Zhong PEI
;
Yuqian TAO
- Publication Type:Journal Article
- Keywords:
Cerebravascular reactivity;
CO2;
Nitric oxide;
Prostaglandins;
Two-photon imaging
- From:
Chinese Journal of Nervous and Mental Diseases
2015;(5):262-266
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine cerebrovascular reactivity to CO2 inhalation in mice. Methods In vivo Two-Pho?ton imaging technique was used to record the reaction of cerebral cortical vessels including penetrating artery, surface vein and capillary in 5 male C57 mice after CO2 inhalation under a thinned-skull cranial window. Nitric oxide syntheses inhibitor L-NAME and Prostaglandin syntheses inhibitor Indomethacin were used to block different vasodilator pathways, respectively. Results Different mouse cortical vessels displayed different degrees of dilation to 1-minute 5%CO2 inhala?tion. The penetrating artery exhibited the most obvious dilation (45.01%±4.45%). L-NAME intervention significantly di?minished cerebravascular CO2 reactivity(P<0.05). Indomethacin significantly attenuated the dilation of artery but not capillary comparing with L-NAME intervention(P<0.05). Conclusions Different vessels react differently to CO2 inhala?tion in which postaglandins and NO signal pathways are involved.
