Cyclosporine A inhibits inflammation and improves the neurological function in a rat model of cerebral isch-emia reperfusion
10.3936/j.issn.1002-0152.2015.05.009
- VernacularTitle:环孢素A抑制炎症改善大鼠脑缺血神经功能
- Author:
Rui WU
;
Shijian LUO
;
Zhendong LI
;
Wenjing QIN
;
Yaning LIU
;
Zhong PEI
- Publication Type:Journal Article
- Keywords:
Cyclosporine A;
Cerebral ischemia reperfusion;
Glial cell;
Neuron;
Neurological function
- From:
Chinese Journal of Nervous and Mental Diseases
2015;(5):299-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the neuroprotective effect of cyclosporine A against cerebral ischemia in a rat model of cerebral ischemia reperfusion. Methods Fifty-two adult male SD rats, weighted 250-280 gram, were randomly divided into three groups: the sham group (group A, n=6), PBS control group (group B, n=23) and cyclosporine A group (group C, n=23). Group C received hypodermic injection of cyclosporine A 10mg/kg daily after surgery and group B re?ceived equal volume of PBS instead. Modified Neurological Severity(mNss)scores were used to assess the neurological deficits at 3, 7, 14, 21 and 30 days following cerebral ischemia. The infarct volume were measured 3 days after reperfu?sion. The neurons, reactive microglia and astrocytes around the infract area were detected by immunofluorescence at 3 and 30 days after surgery. Results Modified Neurological Severity scores were significantly lower in group C than group B at the third(P=0.003),seventh (P=0.011),Fourteenth (P=0.000),twenty-first (P=0.003) and thirtieth (P=0.004) days after surgery. cyclosporine A reduced infarct volume, reactive microglia and astrocytes while increased survived neurons (P<0.001) in ischemic penumbra 3 and 30 days after reperfusion (all P<0.001). Conclusion Continuous injection of cyclosporine A not only protects neurons against ischemia damage but also improves neurological functional recovery af?ter acute stage of damage, possibly through reduction of reactive microglia cells and proliferation of astrocytes.
