Study on Quality Standard for Chidan Ganxian Granules
- VernacularTitle:赤丹肝纤颗粒的质量标准
- Author:
Dongyan CHENG
;
Lishu WANG
;
Donghong CHENG
;
Daqing ZHAO
;
Yonghong LIU
- Publication Type:Journal Article
- Keywords:
Chidan Ganxian granules;
TLC;
HPLC;
Paeoniflorin
- From:
China Pharmacist
2015;(6):1027-1030
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality standard for Chidan Ganxian granules. Methods:TLC was used to identify Paeoni-ae Radix Rubra, Rhizoma Polygoni Cuspidati, Radix Salviae Miltiorrhizae, Herba Saururi and Radix et Rhizoma Rhei in Chidan Ganx-ian granules, and the content of paeoniflorin was determined by HPLC. The stationary phase was an Apollo C18 column ( 150 mm × 4. 6 mm, 5 μm), the mobile phase was acetonitrile-water(12. 5∶87. 5), the flow rate was 1. 0 ml·min-1, the detection wavelength was at 230 nm, and the temperature was 30℃. Results:The characteristic spots of the granules were the same as those of the standard samples without any interference from the negative control. Paeoniflorin had a good linear relationship within the range of 0. 120-1. 436 μg(r=0. 999 4), and the average recovery was 99. 8% with RSD of 1. 80%(n=6). Conclusion:The method is simple, ac-curate, reliable and reproducible, which can be used in the quality control of Chidan Ganxian granules.
