Simultaneous Determination of Hesperidin and Phillyrin in Siji Ganmao Capsules by HPLC
- VernacularTitle:HPLC法同时测定四季感冒胶囊中橙皮苷和连翘苷的含量
- Author:
Lei FAN
;
Min LIU
;
Chaqing WU
;
Weiying WANG
;
Weiping CHEN
- Publication Type:Journal Article
- Keywords:
Siji Ganmao capsules;
Hesperidin;
Phillyrin;
HPLC
- From:
China Pharmacist
2015;(6):1054-1056
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the simultaneous determination of hesperidin and phillyrin in Siji Gamao capsules. Methods:HPLC was applied with the chromatographic conditions as follows:the chromatographic column was Agilent-XDB C18 (250 mm × 4. 6 mm,5μm) at 30℃, the mobile phase was methanol-0. 1% phosphoric acid solution with gradient elution, the flow rate was 1. 0 ml·min-1 , the detection wavelength was 277nm and the injection volume was 5μl. Results:The linear range of hesperi-din and phillyrin was 12.000-60.000 μg·ml-1(r=0.999 6) and 18.000-90.000 μg·ml-1(r=0.999 3),respectively; the average recovery was 96.61%(RSD=1.40%) and 97.66%(RSD=1.27%), respectively. Conclusion: The method is simple, accurate and repeatable, which can be used in the quality control of Siji Ganmao capsules.
