Detection and Identification of Amomum Viosum by Fluorescent Quantitative PCR
- VernacularTitle:阳春砂的荧光定量PCR检测与鉴定
- Author:
Yan YU
;
Weiyang LIANG
- Publication Type:Journal Article
- Keywords:
Amomum viosum;
Fluorescent quantitative PCR;
Identify
- From:
China Pharmacist
2015;(6):946-948
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genetic sequence characteristics of amomum viosum and establish a rapid identification meth-od for amomum viosum by fluorescent quantitative PCR based on DNA analysis. Methods:Amomum viosum and the other samples be-longing to the same genera were collected and identified by experts in the domain. DNA was isolated using commercial kits. The prim-ers and probe were designed according to the conserved region of ITS in amomum viosum. The reaction conditions were optimized to es-tablish the fluorescent quantitative PCR method for the rapid detection of amomum viosum. Results:The fluorescent quantitative PCR method for the rapid detection of amomum viosum was set up. The method could identify amomum viosum successfully, while those samples in the same genera were without amplification curves. Conclusion: Amomum viosum can be identified rapidly by fluorescent quantitative PCR besides the traditional identification by experts.
