Double-index Method for Quality Control of Tribulus Terrestris L
- VernacularTitle:双指标对蒺藜药材质量控制的方法研究
- Author:
Haoyue LI
;
Shuping LIU
;
Xinyue MA
;
Ruihai LI
;
Tianzhu JIA
- Publication Type:Journal Article
- Keywords:
Tribulus terrestris L.;
Saponin;
Total saponins;
Content determination;
HPLC;
Colorimetry
- From:
China Pharmacist
2015;(4):552-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality control method for Tribulus terrestris L. by colorimetry and HPLC. Methods:The HPLC method was with a Welch Ultimate LP-C18 column(250 mm × 4. 6 mm,5μm),the mobile phase was methanol-water(80:20) and the detection wavelength was 203 nm. The colorimetry was with a perchloric acid method. The saponins of Tribulus terrestrist as the index,the determination method for total saponins and saponins of Tribulus terrestris L. was established. Results:The results of the HPLC and colorimetry methods showed saponins of Tribulus terrestris had good linear relationship within the range of 0. 820-7. 380 μg and 24. 600-86. 100 μg with the average recovery of 99. 3% and 99. 5%,respectively. Total saponins and saponins of Tribulus terres-tris in Tribulus terrestris from 18 habitats were measured by the methods. Conclusion:The methods are sensitive,accurate and repro-ducible,and can be used as the quality control methods for Tribulus terrestris.
