Quality Standard for Mongolia Drug Liangxue Shiwei San
- VernacularTitle:蒙成药凉血十味散质量标准研究
- Author:
Rina SHA
;
Chunlong HE
;
Huanyun WANG
;
Shumin LI
- Publication Type:Journal Article
- Keywords:
Liangxue Shiwei San;
Aucklandia Lappa;
Picrorhiza kurrooa Royle ex Benth;
Flos Carthami;
Hydroxysaffor yellow A;
TLC;
HPLC
- From:
China Pharmacist
2015;(5):756-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the identification method for Aucklandia Lappa and Picrorhiza kurrooa Royle ex Benth and the content determination of hydroxysaffor yellow A in Liangxue Shiwei San. Methods:The identification was carried out by TLC. The con-tent of hydroxysaffor yellow A was determined by HPLC. The column was Kormasil C18 (250 mm × 4. 6 mm, 5 μm) and the flow rate was 1. 0 ml·min-1. The mobile phase was methanol-0. 5% acetic acid (30∶70). The detection wavelength was 403 nm and the col-umn temperature was 25℃,and the sample size was 10 μl. Results:The TLC spots were clear with high resolution without interference from the negative sample. A good linearity of hydroxysaffor yellow A was within the range of 1.212-48.480μg·ml-1(r=0.999 8). The average recovery was 98. 13%(RSD=1. 6%,n=6). Conclusion:The established TLC and HPLC methods are simple and accu-rate with good reproducibility, which can be used in the quality control of Liangxue Shiwei San.
