Inhibitory effect of juglone on migration and invasion of glioma cell line U-87 MG
10.3936/j.issn.1002-0152.2015.04.010
- VernacularTitle:胡桃醌对胶质瘤细胞株U-87 MG迁移、侵袭能力的抑制作用
- Author:
Dan YANG
;
Li WANG
;
Zhu LI
;
Zhaoyang LIU
;
Yan SUN
;
Li GUO
;
Rong QI
;
Junping WANG
- Publication Type:Journal Article
- Keywords:
Juglone;
Glioma;
β-catenin;
VEGF;
MMP-2;
MMP-9
- From:
Chinese Journal of Nervous and Mental Diseases
2015;(4):238-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanisms of pin1(peptidyl-prolyl isomerase 1) inhibitor ju?glone on migration and invasion in glioma cell line U-87 MG. Methods Glioma cells were treated with juglone at 0, 0.8, 1.6 and 3.2μmol·L-1. Wound-healing assay and invasion assay were performed to examine the inhibitory activity of ju?glone on glioma cell line U-87 MG. Western blot was used to analyze the protein expression of β-catenin, VEGF, MMP-2 and MMP-9. Results The wound-healing assay showed that the wound-healing rate in juglone-treated groups was 46.04%±6.25%and 30.05%±13.35%at concentrations of 1.6μmol·L-1 and 3.2μmol·L-1 , repectively. Juglone treat?ment significantly reduced the wound-healing rate compared with controls (P<0.05). Transwell invasion assay showed that the number of invaded cells in juglone–treated groups was 103.67 ± 5.69 and 77.33 ± 7.77 at the concentrations of 1.6μmol·L-1 and 3.2μmol·L-1 , respectively. Juglone treatment significantly reduced cell invasion compared with con?trols (P<0.05). Treatment with juglone significantly down-regulated the expression levels ofβ-catenin, VEGF, MMP-2 and MMP-9 in U-87 MG cells in a dose-dependent manner. Conclusion The present data suggests that juglone has a significant inhibitory action on cell migration and invasion through down-regulation of theβ-catenin and its downstream VEGF, MMP-2 and MMP-9 protein expressions in glioma cell line U-87 MG.
