Comparison of Three Methods for Determining Molecular Weight of Sodium Hyaluronate
- VernacularTitle:3种方法测定玻璃酸钠的分子量比较
- Author:
Suting HE
;
Weidong ZHANG
;
Jing LV
;
Cuixia YAN
;
Hong SHAO
;
Gang CHEN
- Publication Type:Journal Article
- Keywords:
Sodium hyaluronate;
Molecular weight;
Viscometry;
SEC;
MALLS-SEC
- From:
China Pharmacist
2015;(6):929-931
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the molecular weight of sodium hyaluronate in the injection determined by three methods. Methods:Viscometry was used to determine the molecular weight of sodium hyaluronate. The molecular weight and its distribution ( Mw/Mn) was measured by size exclusion chromatography ( SEC) and the combination of multi-angle laser light scattering instrument and size exclusion chromatography( MALLS-SEC) . Results:The results of MALLS-SEC were close to those of viscometry, and Mw/Mn was obtained simultaneously. The average value of molecular weight of three batches of samples was 991962, 1007438 and 990232 with RSD of 6. 5%, 4. 6% and 2. 2%, respectively(n=4). The average value of Mw/Mn was 1. 6, 1. 6 and 1. 6 with RSD of 7. 2%, 3. 2% and 3. 2%, respectively(n=4). Conclusion:MALLS-SEC shows better accuracy and precision, and can be used in the quali-ty control of sodium hyaluronate samples.
