Studies on Nephrotoxicity Induced by Triptolide on MDCK Cell Model in vitro
- VernacularTitle:基于狗肾小管上皮细胞的雷公藤甲素体外肾毒性研究
- Author:
Haolan RUAN
;
Qi CHEN
;
Yang LI
;
Zimin XU
;
Sen WENG
- Publication Type:Journal Article
- Keywords:
Triptolide;
MDCK cells;
Toxicity;
Oxidative stress
- From:
China Pharmacist
2015;(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the nephrotoxicity induced by triptolide ( TP) on MDCK cell model and investigate its effect on oxidative stress. Methods:Aristolochic acid was chosen as the positive control. After the MDCK cells were incubated with 0. 5, 5, 50 and 500 nmol·L-1 TP for 24h, MTT method was used to observe the cell inhibiting rate and lactate dehydrogenase (LDH) release test was used to detect the cell membrane damage caused by TP. The cell morphology was observed under an inverted microscope. After the MDCK cells were incubated with 500 nmol·L-1 TP respectively for 30min, 1h, 2h, 4h and 6h, the level of reactive oxygen species ( ROS) was detected using 2′,7′-dichlorodihydro-fluorescein diacetate ( DCFH-DA) as the fluorescent probe. Results:Compared with those of the negative control group, the cell inhibiting rates and the relative LDH release rates in TP-treated group were increased sig-nificantly(P<0. 01). The cells in TP-treated group were creased, turned into the round shape and began to shed off. After the MDCK cells were incubated with TP for 30min, the level of ROS reached the highest value, and then began to decrease (P<0. 01). Conclu-sion:TP can induce the toxic effects on MDCK cells and the mechanism may be related to oxidative stress.
