Estabishment of Identification Method for Longdan Xiegan Pills(Honey Pills) by TLC
- VernacularTitle:龙胆泻肝丸（大蜜丸）薄层色谱鉴别方法的建立
- Author:
Shun ZHAO
;
Hui SUN
;
Ye DING
;
Jinfeng ZHENG
;
Wenli LI
- Publication Type:Journal Article
- Keywords:
Longdan Xiegan pills( honey pills);
TLC;
Quality control
- From:
China Pharmacist
2015;(1):151-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the TLC identification method for Longdan Xiegan pills( honey pills) . Methods:TLC was used in the identification. The samples were extracted by 70% methanol with a heating reflux method, and then extracted by the agents with dif-ferent polarity, including petroleum ether, ethyl acetate and butanol. The petroleum ether part was detected by fluorescence at 365nm for Angelica sinensis, and 1% vanillin-sulfuric acid color reaction was used to detect Alisma orientale. The ethyl acetate part was determined by fluorescence at 365nm for Scutellaria baicalensis, and the butanol part was detected with chloroform-methanol-water (30∶12∶3) as the developing solvent for Bupleurum and Glycyrrhiza, and with acetone-ethyl acetate-water (6∶6∶1) as the developing solvent for gentiopi-croside, geniposide and liquiritin. Results:The developed TLC spots were clear with good separation, high specificity and promising re-producibility. Conclusion:The method can be exactly used in the qualitative identification and quality control of Longdan Xiegan pills ( honey pills) .
