Preliminary Analysis of Tannin-related Constituents in Pomegranate Peel by HPLC-ESI-MS
- VernacularTitle:HPLC-ESI-MS对石榴皮中鞣质类化学成分的初步分析
- Author:
Benhong ZHOU
;
Huilan YI
;
Xianxi GUO
;
Qi FENG
;
Gang LIU
- Publication Type:Journal Article
- Keywords:
HPLC-ESI-MS;
Extracts of pomegranate peel;
Chemical components
- From:
China Pharmacist
2015;(2):201-204
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the tannin-related constituents in the extracts of pomegranate peel by HPLC-ESI-MS. Methods:The separation was performed on a Kromasil C18 column (250 mm × 4. 6 mm,5 μm) with the mobile phase of water and acetonitrile. The flow rate was maintained at 1. 0 ml·min-1 and the detection wavelength was set at 256nm. The samples were analyzed in negative modes. Results:Five tannin-related constituents including gallic acid, punicalin,punicalagin,corilagin and ellagic acid were identified from the extracts of pomegranate peel. Conclusion:The new method is accurate and rapid. It can be used to identify the tannin-related constituents in pomegranate peel and provide new ideas for the research of active components in traditional Chinese medicine as well.
