Simultaneous Determination of Militarine, Protocatechuic Acid and Caffeic Acid in Bletilla Striata by LC-MS/MS
- VernacularTitle:LC-MS/MS法同时测定中药白及中 militarine、原儿茶酸和咖啡酸的含量
- Author:
Li CHEN
;
Chunxia LIU
;
Xiuli HE
;
Xiuhua ZHANG
- Publication Type:Journal Article
- Keywords:
Bletilla Striata;
Militarine;
Protocatechuic acid;
Caffeic acid;
LC-MS/MS
- From:
China Pharmacist
2015;(2):230-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a Q-TRAP LC-MS/MS method for the content determination of militarine, protocatechuic acid and caffeic acid in Bletilla Striata. Methods:A Supelco Discovery C-18 (150 mm × 2. 1 mm, 3 μm) column was used and the mobile phase was acetonitrile-water containing 0. 1% formic acid (v/v) with gradient elution. The flow rate was 0. 25 ml·min-1. Bletilla Striata contrast medicinal materials and Bletilla Striata samples were detected by AB Sciex 4000 Q-MRM scan mode. Results: The content of militarine, protocatechuic acid and caffeic acid in the control herb of Bletilla Striata was 1. 167 5, 0. 062 6 mg·g-1 and 0. 001 0 mg·g-1 , respectively, and that in Bletilla Striata samples was 0. 708 8, 0. 001 1 mg·g-1 and 0. 000 4 mg·g-1 , respec-tively(n=3). Conclusion:The method is simple, accurate and reproducible. It can be used to quantitatively analyze militarine, pro-tocatechuic acid and caffeic acid in Bletilla Striata.
