Study on the HPLC Fingerprint of Dry Ginger
- VernacularTitle:干姜HPLC指纹图谱研究
- Author:
Qi GUO
;
Xiaohong ZHAO
;
Haijian HAN
;
Rui SHEN
;
Ying YANG
;
Hong LEI
;
Yongshen LI
- Publication Type:Journal Article
- Keywords:
Dry ginger;
Fingerprint;
HPLC
- From:
China Pharmacist
2015;(3):397-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC fingerprint of dry ginger to provide experimental evidence for the quality control. Methods:An Agilent TC-C18 column (250 mm × 4. 6 mm,5 μm) was used, the flow phase was acetonitrile-water with gradient elu-tion, the flow rate was 1. 0 ml·min-1 , the column temperature was 30℃, and the detection wavelength was 240 nm. The data were e-valuated by the similarity evaluation software for TCM fingerprint. Results:There were 10 common peaks in HPLC chromatogram of 10 batches of dry ginger at 240 nm, and the chemical similar coefficient was 99%. Conclusion: The fingerprint of ginger at 240 nm is highly specific and typical with a rich fund of information, which can provide useful references for the quality control and evaluation of dry ginger.