Study on in vitro Antimicrobial Activity of Humulus Water Extract
- VernacularTitle:葎草水提液的体外抑菌作用研究
- Author:
Nana ZHANG
;
Zhenwei ZHANG
;
Lei SHI
- Publication Type:Journal Article
- Keywords:
Humulus;
Water extract;
In vitro antibacterial activity
- From:
China Pharmacist
2015;(3):368-370
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the in vitro bacteriostasis of water extract of humulus. Methods:By using double dilution method and 96-well plates trace broth dilution method, the inhibitory test of water extract of humulus was conducted on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The minimum inhibitory concentration ( MIC ) , minimum bactericidal concentration ( MBC) and the diameter of inhibition zone were determined. Results: The drug showed different sensitive degree on the test strains. MIC and MBC for Escherichia coli were 31. 25 mg·ml-1 , that for Staphylococcus aureus were 3. 91 mg·ml-1 , and that for Pseudo-monas aeruginosa was 125 and 250 mg·ml-1 . Conclusion:With the water extract of humulus as the research object, the study on the in vitro bacteriostasis effect of humulus scandens is closer to the clinical application form, which provides reference for the further re-search on the related preparation of humulus scandens.
