IL-17 in the early diagnosis of acute renal allograft rejection in mice
10.3969/j.issn.1672-7347.2011.12.004
- VernacularTitle:IL-17在小鼠肾移植急性排斥反应早期诊断中的作用
- Author:
Ting LI
;
Zhongzhou SI
;
Haizhi QI
;
Zhijun HE
;
Yining LI
- Publication Type:Journal Article
- Keywords:
kidney transplantation;
acute rejection;
T helper cell 17;
interleukin 17
- From:
Journal of Central South University(Medical Sciences)
2011;36(12):1147-1152
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression of T helper (Th) 17 cells and the related interleukin 17 (IL-17) in acute renal allograft rejection in mice and its significance.Methods We established a mouse renal allograft model,in which mice were randomly divided into a renal isograft group and an acute renal allograft rejection group.Three and 7 d after the transplantation,the serum interferon (IFN)-γand IL-17 levels in the mice were determined by enzyme-linked immunosorbent assay,the percentage of Th1 and Th17 cells in the total kidney-infiltrating lymphocytes was investigated by flow cytometry,and the transplanted kidney species were given routine pathological examination after fixation with 10％ formalin.ResultsCompared with the isograft group,the allograft mice showed a significantly higher content of IL-17 (P ＜0.05 ) but not IFN-γ in the serum 3 d after transplantation,and showed significantly higher serum IL-17 and IFN-γcontents 7 d after transplantation (P ＜ 0.05 ).Also,compared with the isograft group,the allograft mice exhibited significantly higher percentage of Th1 and Th17 cells on both day 3 and day 7 ( P ＜ 0.05 ).In the allograft group,the contents of serum IFN-γand IL-17 and the percentage of Th1 and Th17 cells were significantly higher on day 7 than on day 3 (P ＜ 0.05 ).Routine pathological examination indicated that,as time passed,the allograft mice showed gradually stronger rejection responses.ConclusionTh17 cells might play an important role in the development of acute renal allograft rejection,and IL-17 can be used as an early indicator of acute rejection.
