Ischemic preconditioning protects against myocardial ischemia-reperfusion injury through inhibiting toll-like receptor 4/NF-kB signaling pathway in rats
10.3969/j.issn.1672-7347.2011.10.007
- VernacularTitle:缺血预适应通过抑制TLR4/NF-KB信号通路保护大鼠心肌缺血再灌注损伤
- Author:
Jing YANG
;
Yuanjian LI
;
Changping HU
- Publication Type:Journal Article
- Keywords:
toll-like receptor 4;
ischemia/reperfusion injury;
ischemic preconditioning;
heart;
rats
- From:
Journal of Central South University(Medical Sciences)
2011;36(10):972-978
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the protection of ischemic preconditioning (IPC) against myocardial ischemia/reperfusion (I/R) injury is mediated by toll-like receptor 4(TLR4)/NF-kB pathway,and whether these effects are related to the release of calcitonin gene-related peptide (CGRP).Methods Sprague-Dawley rats were subjected to 60 min of ligation of the left anterior descending coronary artery followed by 3 h of reperfusion to induce I/R injury.IPC was performed by 4 cycles of 3-min left coronary artery occlusion followed by 5-min reperfusion before the I/R.The expression of TLR4 mRNA was determined by RT-PCR.TLR4 and NF-kB protein expression were analyzed by immunohistochemistry.Myocardial infarct size,CGRP concentration in plasma and activity of creatine kinase in serum were also measured.Results IPC significantly reduced the infarct size and creatine kinase activity concomitantly with the increase in plasma CGRP concentration.The expressions of TLR4 protein and mRNA and NF-kB protein were increased by myocardial I/R injury,and dramatically inhibited by IPC.Conclusion IPC protects against myocardial I/R injury by inhibition of TLR4/NF-kB pathway.These effects are related to the increased the release of CGRP.
