Calcitonin gene-related peptide suppresses isoprenaline-induced cardiomyocyte apoptosis through regulation of microRNA-1 and microRNA- 133 a expression
10.3969/j.issn.1672-7347.2011.10.006
- VernacularTitle:降钙素基因相关肽通过调节microRNA-1和microRNA-133a的表达抑制异丙肾上腺素诱导的心肌细胞凋亡
- Author:
Jianzhe LI
;
Jun PENG
;
Chenjing WANG
;
Hanwu DENG
;
Yuanjian LI
- Publication Type:Journal Article
- Keywords:
calcitonin gene-related peptide;
isoprenaline;
cardiomyocyte;
apoptosis;
reactive oxygen species;
microRNA-1;
microRNA-133a
- From:
Journal of Central South University(Medical Sciences)
2011;36(10):964-971
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory effect of calcitonin gene-related peptide (CGRP) on cardiomyocyte apoptosis and the underlying mechanism.Methods In cultured rat cardiomyocytes,apoptosis was induced by the incubation of isoprenaline ( 10ˉ5 mol/L) for 48 h.CGRP ( 10ˉ8 or 10ˉ7 mol/L) was administrated for 1 h before incubating isoprenaline to evaluate its effect on cardiomyocyte apoptosis.At the end of the drug treatment,the rate of apoptotic cells and intracellular reactive oxygen species (ROS) were determined,and RNA was extracted to examine the expression of microRNA-1 and microRNA-133a.Results Isoprenaline significantly increased the rate of apoptotic cells and intracellular ROS production concomitantly with the increased microRNA-1 expression and the decreased microRNA-133a expression,which were inhibited by pretreatment with CGRP.The effects of CGRP were reversed by CGRP receptor antagonist.Conclusion CGRP can inhibit the isoprenaline-induced cardiomyocyte apoptosis.The beneficial effect of CGRP is related to regulating microRNA-1 and microRNA-133a expression through the prevention of isoprenaline-induced ROS production.
