Oridonin induced the apoptosis of PC-3 cells and its mechanism

VernacularTitle:冬凌草甲素诱导人雄激素非依赖性前列腺癌PC-3细胞凋亡及其机制
Author: Jin LI ; Luoyan YANG ; Hongtao WU
Publication Type:Journal Article
Keywords: oridonin; PC-3 cell; proliferation; inhibition; apoptosis
From: Journal of Central South University(Medical Sciences) 2011;36(8):754-759
CountryChina
Language:Chinese
Abstract: Objective To observe the proliferation inhibition and apoptosis promotion effect of oridonin on PC-3 cells.Methods PC-3 cells were treated with different concentrations of oridonin.MTT assay and drug concentration-time survival curve were used to test the effect of oridonin on the PC-3 cells.The percentage of earlier apoptosis cells was analyzed by flow cytometry.The protein expression of caspase-3,Bcl-2,and Bax in the PC-3 cells was detected by Western blot.Results Oridonin effectively inhibited the proliferation of PC-3 cells in both concentration- and time-dependent manner,and the IC50 of PC-3 cells was 10.29 μmol/L.Hochest33258 staining and flow eytometry deteced that oridonin induced the apoptosis of PC-3 cells in a concentration-dependent manner (P ＜ 0.05 ).Oridonin down-regulated Bcl-2,up-regulated Bax protein,and activated caspase-3 in a concentration-dependent manner in the PC-3 cells.Conclusion The apoptosis of PC-3 cells induced by oridonin might be associated with the mitochondrial pathway.