miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells
10.3969/j.issn.1672-7347.2011.07.004
- VernacularTitle:miR-149促进鼻咽癌细胞侵袭和上皮-间质转变
- Author:
Zhaohui LUO
;
Liyang ZHANG
;
Zheng LI
;
Chen JIANG
;
Yafei DAI
;
Xiaoping LIU
;
Ying ZHENG
;
Haibo YU
;
Juanjuan XIANG
;
Guiyuan LI
- Publication Type:Journal Article
- Keywords:
miR-149;
NPC;
cell mobility;
invasion and metastasis;
epithelial-mesenchymal transition
- From:
Journal of Central South University(Medical Sciences)
2011;36(7):604-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma (NPC).Methods The expression of miR-149 was examined by real-time PCR and calculated by 2-△△Ct method. The cell proliferation was analyzed by MTT assay. The cell migration and invasion were shown by the wound healing assay and transwell migration assay, and the expression of E-cadherin was detected by Western blot. Results The expression of miR-149 was higher in NPC cell lines 5-8F and 6-10B than that in normal immortalized nasopharyngeal epithelial NP69. MTT assay showed that miR-149 promoted the proliferation of NPC cell lines. The wound healing assay showed miR-149 promoted the mobility and invasion of NPC cell lines. Inhibition of miR-149 reduced the ability of NPC cell lines to proliferate and invade. miR-149 downregulated the expression of E-cadherin, whereas antagomir which mediated knockdown of miR-149 significantly upregulated the expression of E-cadherin. Conclusion miR-149 might be involved in the invasion and metastasis of NPC through regulation of epithelial-mesenchymal transition (EMT).
