Berberine inhibits the invasion and metastasis of nasopharyngeal carcinoma cells through Ezrin phosphorylation
10.3969/j.issn.1672-7347.2011.07.006
- VernacularTitle:黄连素通过抑制磷酸化埃兹蛋白表达阻断鼻咽癌细胞侵袭和转移的机制研究
- Author:
Damao HUANG
;
Weiwei WANG
;
Zhu Feng
;
Lei WANG
;
Yu CHEN
;
Chunlei XIE
;
Jingjing MENG
;
Faqing TANG
- Publication Type:Journal Article
- Keywords:
berberine;
nasopharyngeal carcinoma;
Ezrin/phos-Ezrin;
invasion;
metastasis
- From:
Journal of Central South University(Medical Sciences)
2011;36(7):616-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the molecular mechanism of berberine (BBR) inhibiting the metastasis and invasion of nasopharyngeal carcinoma 5-8F cells, and identify whether berberine suppresses the tumor-invasive action through inhibiting Ezrin or phosphate-Ezrin. Methods The non-cytotoxic concentration of berberine was detected by MTT assay. Filopodia formation of 5-8F cells was observed by electron microscope. The invasion and motility of 5-8F cells with berberine treatment were measured with Trans-well assay. Western blot was used to investigate the Ezrin and phos-Ezrin expression in 5-8F cells treated by berberine. pcDNA3.1-Ezrin and pcDNA3.1-Ezrin M were transfected into 6-10B cells. The inhibitory effect of berberine on the motility and invasion of 6-10B-pcDNA3.1-Ezrin and 6-10B-pcDNA3.1-Ezrin M was detected, respectively. Results Berberine non-cytotoxic concentration was 0-40 μmol/L. After being treated by berberine, filopodia of 5-8F cells obviously reduced, and the permeating artificial basement membrane cells largely decreased in both time- and concentration-dependent manner. There was significant difference compared with the control group (P<0.05). Berberine suppressed the phos-Ezrin expression of 5-8F cells in both time- and concentration-dependent manner (P<0.05), but the effect of berberine was weaker on 6-10B-pcDNA3.1-Ezrin M than on 6-10B-pcDNA3.1-Ezrin. Conclusion Berberine inhibits nasopharyngeal carcinoma cell invasion through inhibiting phos-Ezrin expression and filopodia formation.
