Autologous myoblasts transplantation improves heart function after myocardiac infarction
10.3969/j.issn.1672-7347.2011.04.002
- VernacularTitle:自体成肌细胞移植改善兔心肌梗死后心功能的实验研究
- Author:
Wei ZHUANG
;
Li LI
;
Guoqiang LIN
;
Zhenyu DENG
- Publication Type:Journal Article
- Keywords:
cell transplantation;
myoblast;
myocardiac infarction
- From:
Journal of Central South University(Medical Sciences)
2011;36(4):286-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether autologous skeletal myoblasts implantation improves the cardiac function after myocardial infarction and the possible mechanism. Methods Myocardial infarction was induced by ligation of the left anterior descending coronary artery in rabbits. At 2 weeks, 1. 34 × 107 to 1.75 × 107 autologous skeletal myoblasts were infused into the lesion via direct intramuscular injection. In the control group, the postinfarction hearts were infused with medium alone. Buxco invasive cardiac function testing and histopathological examination were utilized to evaluate the functional and structural changes in the myocardium 4 weeks later. Results Both maximum rising rate of the left intraventricular pressure [+dp/dtmax,( 1 217.77 +89.91 )mmHg/s vs. (897.83 ±70.04) mmHg/s] and maximum falling rate of the left intraventricular pressure [- dp/dtmax,( -1174.58 ± 91.5 ) mmHg/s vs. ( - 753.67 ± 69.66 ) mmHg/s] were improved in the myoblast transplanted group compared with medium infusion group. The positive desmin immunostaining skeletal myofibers in the myocardium were found throughout the infracted areas and the border zone. Conclusion Autologous skeletal myoblasts can establish muscle tissue when transplanted into postinfarction hearts, and this mucle can treat myocardiac infarction effectively.
