Nerve growth factor induced expression of iNOS and substance P in dorsal root ganglion sensory neuron and interferon regulatory factor-1
10.3969/j.issn.1672-7347.2011.05.003
- VernacularTitle:神经生长因子诱导背根节感觉神经元细胞iNOS和P物质的表达及干扰素调节因子-1的作用
- Author:
Pinhua PAN
;
Siyun HUANG
;
Chengping HU
- Publication Type:Journal Article
- Keywords:
airway;
neurogenic inflammation;
nerve growth factor;
interferon regulatory factor-1
- From:
Journal of Central South University(Medical Sciences)
2011;36(5):386-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of airway neurogenic inflammation by studying the expression of inducible nitric oxide synthase (iNOS) and substance P in C7-T5 dorsal root ganglion sensory neuron cells induced by nerve growth factor (NGF) and the role of interferon regulatory factor-1 (IRF-1).Methods The dorsal root ganglia neuron (DRGn) cells were primary cultured,and then stimulated with or without NGF or NGF+interferon (IFN)-γ.Subsequently the DRGn cells were transinfected with or without green fluorescent protein (GFP)-IRF-1-vshRNA,and then stimulated with or without NGF.The expressions of iNOS and substance P were detected by real-time PCR.Results NGF induced the mRNA expression of iNOS and substance P in dorsal root ganglion sensory neuron cells,and IFN-γ increased NGF-induced iNOS mRNA expression.The expressions of iNOS and substance P in sensory neuron cells were decreased significantly at the mRNA level after IRF-1 was blocked down by IRF-1-vshRNA transinfection.Conclusion NGF is involved in the airway neurogenic inflammation by prompting the expression of iNOS and substance P through transcription factor IRF-1 in airway sensory neuron cells.IRF-1 may be a therapeutic target for airway neurogenic inflammation.
