Detection of CTP gene expression in the asexual erythrocytic stages of Plasmodium falciparum (FCC1 /HN) by reverse transcriptase- polymerase chain reaction and construction of eukaryotic expression vector of CTP gene
- VernacularTitle:利用RT-PCR的方法检测恶性疟原虫海南株FCC1/HN CT基因在红细胞内期的表达及CTP基因真核表达载体的构建
- Author:
Huihong CHEN
;
Xinbing YU
;
Zhongdao WU
;
Jin XU
;
Jiahai LU
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum;
reverse transcriptase - polymerase chain reaction (RT - PCR);
CTP(phosphocholine cytidylyltransferase) cDNA
- From:
Journal of Tropical Medicine
2001;1(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
Objectve To detect whether the CTP(phosphocholine cytidylyltransferase) gene was expressed in the asexual erythrocytic stages of Plasmodium falciparum (FCC 1/HN )by using the RT - PCR and to construct eukaryotic expression vector of CTP. Method The erythrocytic stage parasites of Plasmodium falciparum were cultured as described by Trager and Jensen. RNA from erythrocytic stage parasite was extracted by using Trizol reagent. The complete genes coding for CTP gene isolates FCCI/HN were amplified by reverse transcriptase -polymerase chain reaction(RT- PCR). CTP gene was cloned into eukaryotic expression vector pcDNA3. Results CTP encoding gene was amplified from the erythrocytic stages of Plasmodiumfalciparum (FCC 1/HN) and eukaryotic expression vector of CTP was constructed. Conclusion CTP gene was expressed in the erythrocytic stages of Plasmodium falciparum (FCC 1/HN) and eukaryotic expression vector of CTP was successfully constructed.
