The role of human fibroblast growth factor receptor 1-Ⅲb isoform in pancreatic cancer cells
- VernacularTitle:人类成纤维细胞生长因子受体1的Ⅲb亚型在胰腺癌细胞中的作用
- Author:
Zhanbing LIU
;
Yinmo YANG
;
Qilu QIAO
;
Jianxun ZHAO
;
Yanting HUANG
;
Marko Kornmann
- Publication Type:Journal Article
- Keywords:
pancreatic neoplasms;
receptors,fibroblast growth factor;
transfection
- From:
Medical Journal of Chinese People's Liberation Army
2007;32(2):88-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of Ⅲb isoform of human fibroblast growth factor receptor 1 (FGFR1-Ⅲb) in PANC-1 pancreatic cancer cells. Methods The plasmid of human full-length FGFR1-Ⅲb isoform，pSVK4/FGFR1-Ⅲb, was stable transfected into cultured PNAC-1 pancreatic cancer cell lines facilitated by lipofectamine. The function of FGFR1-Ⅲb in transfected pancreatic cancer cells were examined by MTT assay, soft agar assay, cell migration assay, single cell movement assay, In vivo tumorigenicity assay. Results The basal anchorage-dependent and -independent cell growth was significantly inhibited. Additionally, FGFR1-Ⅲb expression inhibited single cell movement and in vitro invasion as determined by time-lapse microscopy and boyden chamber assay as well as in vivo tumor formation and growth in nude mice. Microscopic analysis of the xenograft tumors revealed a reduced Ki-67 labelling, lower amount of tumor necrosis and higher grade of differentiation in FGFR1-Ⅲb expressing tumors. Conclusion We identified a functional human FGFR mRNA splice variant that inhibits the transforming potential of pancreatic cancer cells.
