Effect of melatonin on the balance of ET-1 and eNOS in the renal cortex of insulin-resistant rats
- VernacularTitle:褪黑素对胰岛素抵抗大鼠肾皮质内皮素、内皮型一氧化氮合酶平衡的影响
- Author:
Ying LIU
;
Zhimin LIU
;
Ying ZHAO
;
Jin HE
;
Huimin LIU
;
Ling MENG
- Publication Type:Journal Article
- Keywords:
melatonin;
insulin resistance;
ET-1;
eNOS;
kidney cortex
- From:
Medical Journal of Chinese People's Liberation Army
2007;32(9):912-915
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of melatonin on the expression of endothelin-1 (ET-1) and endothelial nitricoxide synthase (eNOS) in renal cortex of insulin-resistant (IR) rats. Methods Insulin-resistant rat model was established with 6-8 week ages Sprague-Dawwley (SD) rats by high-glucose diet (70% calories from glucose) for 6 weeks. IR rats models were divided into control normal group (CN, n=10), IR group (n=10) and melatonin group (MEL, n=10). Rats in MEL were affused melatonin 10mg/(kg·d) (4PM) for further 6 weeks. The protein and mRNA in rats' renal cortex of each group were assayed by immunohistochemistry and RT-PCR. Results Arterial blood pressure (ABP) and serum triglyceride (TG), free fatty acid (FFA), malondialdehyde (MDA), insulin (Ins) and homeostasis model analysis insulin resistance (HOMA-IR) in MEL were lower than that in IR (all P＜0.01). Serum high-density lipoprotein (HDL-c) and superoxide dismutese (SOD) were higher in MEL than that in IR (all P＜0.01). HE staining showed that the structure of renal cortex in MEL is approximately normal. The levels of ET-1 protein and mRNA in renal cortex of MEL rats were lower than that in IR group. The levels of eNOS protein and mRNA of renal cortex in MEL group were higher than that in IR group (all P＜0.05). Conclusion At the early stage of IR, melatonin application can ameliorate the imbalance between ET-1 and eNOS and protect the angio-endothelial function of renal cortex in high-glucose induced IR rats.
