An improved method to recover high quality genomic DNA from paraffin-embedded tissues
- VernacularTitle:一种从石蜡包埋组织中获取高质量基因组DNA的改良方法
- Author:
Yilike XIAMIXINUER
;
Xi YANG
;
Guizhen WU
;
Qi MA
;
Abudula ABULIZI
- Publication Type:Journal Article
- Keywords:
DNA extraction;
paraffin-embedded tissue;
PCR
- From:
Journal of Central South University(Medical Sciences)
2009;34(10):1042-1046
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high quality genomic DNA preparation method from formalin fixed and paraffin embedded tissues (FFPET) by integrating previous DNA extraction methods. Methods We combined classical genomic DNA extraction methods and commercially available DNA affinity column, replaced the de-waxing by dimethylbenzene with water-bath, designed a fast and improved genomic DNA preparation method. We also extracted genomic DNA from paraffin embedded cervical cancer tissues, and checked the quality of DNA by agarose gel electrophoresis and polymerase chain reaction detection. Results The improved genomic DNA extraction method combined the advantages of the water-bath de-waxing and DNA affinity column, making it possible to get high quality genomic DNA from paraffin embedded cervical cancer tissues, and especially efficient to recover genomic DNA fragments larger than 20 kb. Conclusion The improved DNA extraction method is fast and convenient to recover high quality genomic DNA from paraffin embedded tissues.
