Effect of oxaliplatin on the survival rate of human Y79 cells after down-regulation of Mcl-1
10.3980/j.issn.1672-5123.2017.12.09
- VernacularTitle:siRNA下调Mcl-1后奥沙利铂对人Y79细胞凋亡率的影响
- Author:
Zhou LU
1
;
Li NA
Author Information
1. 315026,中国浙江省宁波市眼科医院药剂科
- Keywords:
retinoblastoma;
oxaliplatin;
Mcl - 1;
apoptosis
- From:
International Eye Science
2017;17(12):2226-2228
- CountryChina
- Language:Chinese
-
Abstract:
·AIM: To study the effect of oxaliplatin on the survival rate of Y79 after down-regulation of Mcl-1 by SiRNA.·METHODS: Y79 cells were cultured in RPMI1640. The cultured cells were stimulated with 0. 25μmol/L of oxaliplatin. The expression of Mcl - 1 protein was detected by Western blot after 6, 16 and 24h respectively. Cells in logarithmic phase were collected and used for single-cell suspension. Then they were transfected with empty plasmid, Mcl-1-homo-991, Mcl-1-homo-1114 and Mcl - 1 - homo - 1235. After 6h, fluorescence microscope was used to observe the transfection efficiency and the optimal one was selected. The cells were divided into Group A and transfected with empty plasmids. The cells transfected with Mcl-1 were divided into Group B and Group C. Group A and Group C were treated with 0. 25μmol/L oxaliplatin for stimulating induction, and the apoptotic rate was compared after 24h.·RESULTS: The expression of Mcl-1 in Y79 stimulated by oxaliplatin was the most after 24h of culture. Mcl-1-homo-991 significantly inhibited the expression of Mcl-1 in Y79 after transfection. There was no significant difference in the apoptosis rate in Group A ( 11. 1% ± 1. 2%) and in the control group (6. 1%±0. 6%)(P>0. 05). The apoptotic rate of Group C ( 49. 2% ± 2. 7%) was significantly higher than that of Group B (20. 8%±1. 9%).At the same time, the apoptotic rates of these two groups were significantly higher than those of Group A and control group, the difference was statistically significant (P<0. 05).·CONCLUSION:Downregulation of Mcl-1 by siRNA can reduce the drug resistance of Y79, thereby enhancing the apoptosis of Y79, and reducing the survival rate of Y79.
