Epicutaneous sensitization with ovalbumin, staphylococcal enterotoxin B and vitamin D analogue induces atopic dermatitis in mice
10.11817/j.issn.1672-7347.2017.09.005
- VernacularTitle:卵清蛋白、金黄色葡萄球菌肠毒素B和维生素D类似物诱导小鼠特应性皮炎模型
- Author:
Lina TAN
1
;
Jianyun LU
;
Meilin CHEN
;
Yaping XIANG
;
Qingmei CHENG
;
Yunsheng LIANG
;
Jinhua HUANG
;
Jian HUANG
;
Jing CHEN
;
Lihua GAO
Author Information
1. 中南大学湘雅三医院皮肤科
- Keywords:
atopic dermatitis;
vitamin D analogue;
BALB/c mice;
inflammatory cytokines;
IgE;
epidermal thickness
- From:
Journal of Central South University(Medical Sciences)
2017;42(9):1023-1029
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To illuminate a method for establishment of a cost-efficient atopic dermatitis (AD) mouse model by topical application of ovalbumin (OVA),super-antigen staphylococcal enterotoxin B (SEB),and calcipotriene ointment (CO) on the back of BALB/c mice.Methods:Experimental mice were topically treated with OVA/SEB or OVA/SEB/CO every other day during 15 days of induction.Clinical alterations on the skin area were monitored every other day.Epidermal thickness were measured by reflectance confocal microscope (RCM) before harvest.Inflammatory cells in skin biopsies were marked by hematoxylin-eosin (HE) staining.Blood sample and skin biopsies were measured by ELISA and quantitative real-time PCR to detect the expression of IL-2,IL-4,IL-31,interferon (IFN)-γ,tumor necrosis factor (TNF)-α pruritus-associated nerve growth factor (NGF),and serum IgE.Results:Human AD-like cutaneous local inflammatory reaction was characterized by the accumulation of inflammatory cells,increased epidermal thickness and serum IgE levels as well as Th1 cell-associated cytokines (IFN-γ,TNF-α),Th2 cell-associated cytokines (IL-4,IL-31),and NGF in the OVA/SEB/CO group compared with that in the normal control group or the OVA/ SEB group.Conclusion:OVA/SEB/CO can induce an AD-like mouse model with lower economic and time consumption.
