Establishing mouse model of type 2 diabetes peripheral neuropathy and measuring its intra-epidermal nerve fiber density
- VernacularTitle:2型糖尿病小鼠周围神经病变模型的建立及皮下神经纤维密度的检测
- Author:
Jiangpan PU
1
;
Jiawen TANG
;
Tao ZHU
Author Information
1. 201600,南京医科大学附属上海市松江区中心医院麻醉科
- Keywords:
Type 2 diabetes;
Diabetic peripheral neuropathy;
Mouse model;
Intra-epidermal nerve fiber density
- From:
The Journal of Clinical Anesthesiology
2017;33(10):1006-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish mouse model of type 2 diabetes peripheral neuropathy and measure its intra-epidermal nerve fiber density (IENFD).Methods Male C57BL6 mouse were ran-domly divided into four groups:group HS (n =6):high-fat diet+single streptozotocin intraperitoneal injection (120 mg/kg);group H (n =6):high fat diet+buffer injection;group S (n =6):standard chow diet+single streptozotocin intraperitoneal injection (120 mg/kg);group C (n = 6 ):standard chow diet+buffer injection.The 24th week was the end point of the experiment,and random glucose, homeostasis model assessment of insulin resistance (HOMA-IR),mechanical threshold,and IENFD were measured.Results Group HS had significantly higher random glucose and HOMA-IR than other groups (P <0.01),had significantly lower mechanical threshold than other groups (P <0.05), had significantly lower IENFD than groups S and C at 24th week (P <0.05 );group H had signifi-cantly higher random glucose and HOMA-IR than group C at 24th week (P <0.01),had no signifi-cant difference in mechanical threshold compared with group S and group C,and had no significant difference in IENFD compared with group HS.Conclusion A mouse model of type 2 diabetes periph-eral neuropathy was successfully established,and the IENFD was found to be decreased significantly.
