Effects of sevoflurane exposure on the hypothalamic-pituitary-adrenal axis develoopment in neonatal rat
- VernacularTitle:七氟醚对新生大鼠下丘脑-垂体-肾上腺皮质轴发育的影响
- Author:
Ju ZOU
1
;
Yan LIU
;
Sijie TAN
Author Information
1. 南华大学医学院寄生虫学教研室
- Keywords:
Sevoflurane;
Hypothalamic-pituitary-adrenal axis;
Bumetanide;
Stress
- From:
The Journal of Clinical Anesthesiology
2017;33(10):1021-1024
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sevoflurane exposure on the hypothalamic-pituitary-adrenal (HPA)axis development in neonatal rats.Methods Fifty-four SD rats (P6)were randomly divided into three groups:control group (group C),sevoflurane group (group S)and bu-metanide+sevoflurane group (group BS),n = 18 in each group.In group C,rats were treated with pure oxygen for 6 hours and rats in group S received 2.1% sevoflurane anesthesia for 6 h.Rats in group BS also received 2.1% sevoflurane anesthesia for 6 h,but they were injected with bumetanide before 30 min and at 3 h during the anesthesia.6 rats from each group were randomly selected and the tail blood was collected to measure the corticosterone in the serum using ELISA.Remaining rats in each group were normally housed to P60.A elevated plus maze (EPM)were employed to investigate the anxiety-like behavior,and restraint stress were used to evaluate the function of HPA axis. Results In P6 rats,sevoflurane significantly increased the serum corticosterone level (P < 0.01 ) while bumetanide pretreatment decreased corticosterone level (P <0.01).In P60 rats,neonatal expo-sure to sevoflurane decreased the visits and time spent in the open arms of EPM (P < 0.05 ),in-creased the corticosterone level pre-stress and post-stress (P <0.05 or P < 0.01 ).Pretreatment of bumetanide normalized serum corticosterone level and the anxiety behavior in the EPM. Conclusion Neonatal sevoflurane exposure caused hyperactive HPA axis in rats,which is normalized by bumetanide pretreatment through blocking GABAA receptors.
