TNF-αpromotes apoptosis of rat islet βcell line
10.3969/j.issn.1001-6325.2017.12.003
- VernacularTitle:TNF-α促进大鼠胰岛β细胞系凋亡
- Author:
nan Nan LIANG
1
;
Qian ZHANG
;
zheng Xiang WU
;
hui Jia ZHAO
;
Jin WANG
;
ying Xiang JIAO
Author Information
1. 山西医科大学生理学系
- Keywords:
TNF-α;
FOXO1;
ChREBP;
TXNIP;
INS-1 cells
- From:
Basic & Clinical Medicine
2017;37(12):1668-1673
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of tumor necrosis factor-α( TNF-α) on islet cell apoptosis and TXNIP expression.Methods INS-1 cells were cultured in vitro, treating with TNF-α(0, 1, 5, 10 and 20 mg/L).We tested the effect of TNF-αon cell viability by CCK-8.INS-1 cells were treated with TNF-α( 5 mg/L, 24 h) for the proper concentration and incubation time; mRNA expression of TXNIP and ChREBP were measured by real-time PCR;in addition, protein levels of TXNIP , ChREBP and FOXO1 were analyzed by Western blot .Results TNF-αdecreased the survival rate of INS-1 cells in a dose-dependent manner ( P<0.05 ) , and induced apoptosis;protein and mRNA expression of TXNIP and ChREBP were significantly higher than that in control group ( P<0.05 );while the expression of protein level of FOXO 1 was down-regulated .Conclusions TNF-αinduces apoptosis in INS-1 cells and aggravates the cells damage .
