miR-29c promotes apoptosis in prostate cancer cell line PC 3
10.3969/j.issn.1001-6325.2017.12.009
- VernacularTitle:miR-29c促进前列腺癌PC3细胞凋亡
- Author:
jing Nan LIU
1
;
Ting LI
;
Yan ZHAO
;
ni Yan HAO
;
hou Xiao WU
;
li Chun LUO
Author Information
1. 重庆医科大学检验医学院临床检验诊断学教育部重点实验室
- Keywords:
prostate cancer;
miR-29c;
VEGF;
VEGFR2;
apoptosis
- From:
Basic & Clinical Medicine
2017;37(12):1706-1711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of miR-29c on the apoptosis of prostate cancer cell line PC 3.Methods The expression of miR-29c, VEGF and VEGFR2 in RWPE-1 and PC3 were detected to verify the different between normal and cancer cell .The location of p-VEGFR2 was measured by immunofluorescence (IF).After PC3 cell were transfected by miR-29c overexpression adenovirus , the expression level of miR-29c, VEGF, t-VEGFR2, p-VEGFR2, BAX and Bcl-2 were measured by RT-PCR and Western blot , the cell apoptosis rate was detected by the kits of ho-echst 33258 and FCM.Results The expression level of miR-29c was lower and VEGF, VEGFR2 was higher in PC3 cell compared with that in RWPE-1(P<0.001).The expression level of miR-29c and BAX were higher and VEGF , p-VEGFR2 and Bcl-2 were lower in Ad-miR-29c group compared with that in control group (P<0.001).Higher apop-tosis rate was detected in Ad-miR-29c group compared with control group (P<0.001).Conclusions miR-29c can promote apoptosis in prostate cancer cell PC 3 by significantly inhibiting VEGF/VEGFR2 signaling.
